Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase
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- 北京华夏远洋科技有限公司 商铺
- 认证
- 报价
- 请来电询价
- 性状
- 试剂
- 适应症
- 科研试验
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- 北京市丰台区造甲街110号2号平房(新村企业集中办公区)
- 联系电话
- 010-62898668
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产品详细介绍
主要组成成分 | Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase | 性状 | 试剂 |
适应症 | 科研试验 | 规格 | 50000u |
有效期 | 36(月) | 生产企业 | genscript |
药(械)准字 | Genscript Z02032 |
cat. no.
name
size
price
selected
z02032
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moloney murine leukemia virus (m-mlv) reverse transcriptase |
10,000u
|
$39.00
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abbreviated name-1
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m-mlv rt |
documentsfiguresreference
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description
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genscriptmoloney murine leukemia virus (m-mlv) reverse transcriptaseis an rna-dependent dna polymerase that uses single-stranded rna, dna, or an rna-dna hybrid (using a primer) to synthesize a complementary dna strand. the enzyme lacks 3 -5 exonuclease activity. | |||||||
source
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e. coli | |||||||
purity
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purity is > 90% as determined by sds-page. | |||||||
reaction buffer
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5x m-mlv rt reaction buffer: 250 mm tris-hcl (ph 8.3), 375 mm kcl, 15 mm mgcl2, and 50 mm dtt. | |||||||
storage
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store the protein at -20°c. avoid exposure to frequent temperature changes. | |||||||
unit definition
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one unit is defined as the amount of enzyme required to
catalyze the transfer of 1 nmol of deoxynucleotide into
acid-precipitable material in 10 minutes at 37°c. the reaction conditions are as follows: 50 mm tris-hcl (ph 8.3), 7 mm mgcl2, 40 mm kcl, 10 mm dtt, 0.1 mg/ml bsa, 0.5 mm [3h]dttp, 0.025 mm oligo (dt), 0.25 mm poly(a), and 0.01% np-40. |
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concentration
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200,000 units/ml in 20 mm tris-hcl (ph 7.5), 200 mm nacl, 1 mm dtt, 0.01% np-40, 0.1 mm edta, 50% glycerol | |||||||
control
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activity testing shows no detectable dnase or rnase contamination. |
1
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custom recombinant protein services:guaranteede. coliexpression package-3 mg purified soluble protein from $2,200 . |
2
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bacterial expression system: genscript has developedbacpowertmtechnologies to tackle hard-to-express and hard-to-dissolve proteins. |
3
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yeast expression system: genscript"s proprietaryyeasthightmtechnology combines the advantages of the bacterial and eukaryotic expression systems. |
4
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baculovirus/insect cell expression system: genscript developedbacuvancetmplatform for the secretion of recombinant proteins from baculovirus-infected insect cells. |
5
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mammalian cell expression system: the proprietary 293 and cho suspension system platform promotes fast production and delivery of recombinant proteins and monoclonal antibodies up to grams level. |
主要经营:Axygen实验耗材 ; Corning实验耗材 ; Sigma生化试剂及抗体期货8折
北京华夏远洋科技有限公司位于北京市,是一家Axygen实验耗材、Corning实验耗材、Sigma生化试剂及抗体、BD生命科学产品、Abcam抗体、Gbico、Hyclone血清、接受抗体定制、免疫组 ...
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