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Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase

信息编号:261819 浏览:531次
供应商
北京华夏远洋科技有限公司 商铺
认证
报价
请来电询价
性状
试剂
适应症
科研试验
所在地
北京市丰台区造甲街110号2号平房(新村企业集中办公区)
联系电话
010-62898668
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15201195988
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产品详细介绍

主要组成成分 Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase 性状 试剂
适应症 科研试验 规格 50000u
有效期 36(月) 生产企业 genscript
药(械)准字 Genscript Z02032
cat. no.
name
size
price
selected
z02032
moloney murine leukemia virus (m-mlv) reverse transcriptase
10,000u
$39.00
full namemoloney murine leukemia virus (m-mlv) reverse transcriptase
abbreviated name-1
m-mlv rt documentsfiguresreference
msds: 20081030012707(pdf)
coa: z02032_r211906(pdf)
description
genscriptmoloney murine leukemia virus (m-mlv) reverse transcriptaseis an rna-dependent dna polymerase that uses single-stranded rna, dna, or an rna-dna hybrid (using a primer) to synthesize a complementary dna strand. the enzyme lacks 3 -5 exonuclease activity.
source
e. coli
purity
purity is > 90% as determined by sds-page.
reaction buffer
5x m-mlv rt reaction buffer: 250 mm tris-hcl (ph 8.3), 375 mm kcl, 15 mm mgcl2, and 50 mm dtt.
storage
store the protein at -20°c. avoid exposure to frequent temperature changes.
unit definition
one unit is defined as the amount of enzyme required to catalyze the transfer of 1 nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37°c.
the reaction conditions are as follows: 50 mm tris-hcl (ph 8.3), 7 mm mgcl2, 40 mm kcl, 10 mm dtt, 0.1 mg/ml bsa, 0.5 mm [3h]dttp, 0.025 mm oligo (dt), 0.25 mm poly(a), and 0.01% np-40.
concentration
200,000 units/ml in 20 mm tris-hcl (ph 7.5), 200 mm nacl, 1 mm dtt, 0.01% np-40, 0.1 mm edta, 50% glycerol
control
activity testing shows no detectable dnase or rnase contamination.
1
custom recombinant protein services:guaranteede. coliexpression package-3 mg purified soluble protein from $2,200 .
2
bacterial expression system: genscript has developedbacpowertmtechnologies to tackle hard-to-express and hard-to-dissolve proteins.
3
yeast expression system: genscript"s proprietaryyeasthightmtechnology combines the advantages of the bacterial and eukaryotic expression systems.
4
baculovirus/insect cell expression system: genscript developedbacuvancetmplatform for the secretion of recombinant proteins from baculovirus-infected insect cells.
5
mammalian cell expression system: the proprietary 293 and cho suspension system platform promotes fast production and delivery of recombinant proteins and monoclonal antibodies up to grams level.
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