Angel DNA Polymerase

主要组成成分	Angel DNA Polymerase	性状	试剂
适应症	科研试验	规格	500u
有效期	36（月）	生产企业	genscript
药（械）准字	genscript Z02033

name selected

l00339	cloneez? pcr cloning kit	24rxns	$175.00

cloneez® pcr cloning kit description

the genscriptcloneez®pcr cloning kitis designed for the quick pcr cloning , it is especially powerful in high-throughput cloning of pcr products into any destination vector effectively by sidestepping the tedious and limiting tasks such as selecting proper restriction enzymes, phosphatases, or ligases. using our proprietary cloneez®enzyme, this kit is able to rapidly generate precise and directional constructs by carrying out 30-minute incubation at room temperature (20 °c-25 °c).

key features

the cloneez®pcr cloning kit offers flexible and quick directional cloning and enables users to::

clone into any vector without any need for restriction enzymes, phosphatase treatments, or ligases provide up to 12 kb pcr cloning at any restriction site rapidly and precisely generate correctly oriented constructs and inserts work with pcr that employ any thermostable polymerases

applications the genscript cloneez®pcr cloning kit can be used in a variety of applications: pcr cloning htp pcr cloning joining of dna fragments
the inserts were detected by pcr screening using single colonies. some of the results are shown in the figure below.


technical manual download pdf filetm0279
components componentspuc57 linearized (kpni/hindiii,100 ng/μl)1-kb control insert (100 ng/μl)10x cloneez?reaction buffercloneez?enzyme (5 u/μl)

l00339

10 μl

10 μl

100 μl

50 μl

document msds:20081029013928(pdf)