M-MLV Reverse Transcriptase(Rnase H-)
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- 北京华夏远洋科技有限公司 商铺
- 认证
- 报价
- 请来电询价
- 性状
- 试剂
- 适应症
- 科研试验
- 所在地
- 北京市丰台区造甲街110号2号平房(新村企业集中办公区)
- 联系电话
- 010-62898668
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- 15201195988
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- 朱少雄 请说明来自顺企网,优惠更多
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产品详细介绍
主要组成成分 | M-MLV Reverse Transcriptase(Rnase H-) | 性状 | 试剂 |
适应症 | 科研试验 | 规格 | 10000u |
有效期 | 36(月) | 生产企业 | genscript |
药(械)准字 | genscript Z02040 |
l00204
|
one-hour westerntmbasic kit (rabbit) |
1kit (5 assays)
|
$60.00
|
|
genscript"s one-hour westerntmkit yields journal-
western or dot blot results in about one hour. using genscript"s
breakthrough immunodetection technology (patent pending), the kit
replaces the classical three-step western process, which take
nearly five hours. transfer the proteins from gel to membrane and
incubate it in the pretreat solution for five minutes. then
incubate in wb-2 solution with the mixture of primary antibody and
wb-1 solution for 40 minutes. then wash three times for five or ten
minutes each. the membrane can then be developed with the hrp
substrate. the one-hour westerntmprocedure is contrasted
with a classical three-step western at right.
the one-hour westerntmkit is designed to produce high
signal with low background for quick and clear western analysis of
proteins.
except for basic kits, all other kits contain all the necessary
reagents, buffers and materials such as membrane for performing a
western blot, and no additional secondary antibody is needed.
the one-hour westerntmkit saves precious research time.
for added cost-efficiency, we recommend our complete kits, which
contain all the reagents, membrane, hrp substrate necessary for a
perfect blot. however, the kit components are also sold
separately.
comparison of one-hour westerntmblot detection
with classical western blot detection.
one-hour westerntmblot detection was compared with
classical western blot detection of α-tubulin protein from hela
cell lysate. serially diluted hela cell lysate samples were western
blotted to westcleartmnitrocellulose membrane
(genscript, l00224) after sds-page. the membrane was then cut into
two halves and processed with different procedures using α-tubulin
monoclonal antibody (abcam, ab7291): one-hour
westerntmblot detection (1 hr, right panel of figure 2)
and classical western-blot detection (4.5 hours, left panel of
figure 2).
figure 2. western blots for the detection of α-tubulin protein from
hela cell lysate by both classical western and one-hour
westerntmbasic kit. 10 μg, 2.5 μg, 0.63 μg, and 0.16 μg
of hela cell lysate (bd biosciences, 611449) were loaded in
different lanes as shown in the figure. the classical western blot
was developed with ecl system (ge healthcare, rpn2109). the
one-hour western blot was developed with
lumisensortmchemiluminescent hrp substrate kit
(genscript, l00221v60).
(0.2 μm, 7.5 x 8 cm)5x wash solutionchromosensortmone solution tmb substrate
l00204 | l00205 | l00399 |
x | x | x |
50 ml | 50 ml | 50 ml |
50 ml | 50 ml | 50 ml |
1 | 1 | 1 |
0.5 ml | 0.5 ml | 0.5 ml |
50 ml | 50 ml | 50 ml |
x | x | x |
125 ml | 125 ml | 125 ml |
x | x | x |
l00204t
|
one-hour westerntmstandard kit using tmb (rabbit) |
1kit (5 assays)
|
$75.00
|
|
l00205t
|
one-hour westerntmstandard kit using tmb (mouse) |
1kit (5 assays)
|
$75.00
|
|
l00224a60
|
westcleartmnitrocellulose membrane |
1pk (5 sheets of 7.5 x 8 cm)
|
$20.00
|
|
l00221v60
|
lumisensortmchemiluminescent hrp substrate kit |
1kit (2 x 30ml)
|
$55.00
|
|
l00222v60
|
chromosensortmone-solution tmb substrate |
1pk (60ml)
|
$28.00
|
|
l00228
|
one-hour westerntmstandard kit (goat) |
1kit (5 assays)
|
$75.00
|
|
l00205
|
one-hour westerntmbasic kit (mouse) |
1kit (5 assays)
|
$60.00
|
|
l00204c
|
one-hour westerntmstandard kit (rabbit) |
1kit
|
$75.00
|
|
l00205c
|
one-hour westerntmstandard kit (mouse) |
1kit
|
$75.00
|
|
l00399
|
one-hour westerntmbasic kit (goat) |
1kit (5 assays)
|
$60.00
|
|
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