X-Gal/IPTG Premix

主要组成成分	X-Gal/IPTG Premix	性状	试剂
适应症	科研试验	规格	5ml
有效期	36（月）	生产企业	genscript
药（械）准字	genscript M00092

name selected

l00204	one-hour westerntmbasic kit (rabbit)	1kit (5 assays)	$60.00

one-hour westerntmbasic kit (rabbit) kit description

genscript"s one-hour westerntmkit yields journal- western or dot blot results in about one hour. using genscript"s breakthrough immunodetection technology (patent pending), the kit replaces the classical three-step western process, which take nearly five hours. transfer the proteins from gel to membrane and incubate it in the pretreat solution for five minutes. then incubate in wb-2 solution with the mixture of primary antibody and wb-1 solution for 40 minutes. then wash three times for five or ten minutes each. the membrane can then be developed with the hrp substrate. the one-hour westerntmprocedure is contrasted with a classical three-step western at right.

the one-hour westerntmkit is designed to produce high signal with low background for quick and clear western analysis of proteins.

except for basic kits, all other kits contain all the necessary reagents, buffers and materials such as membrane for performing a western blot, and no additional secondary antibody is needed.

the one-hour westerntmkit saves precious research time. for added cost-efficiency, we recommend our complete kits, which contain all the reagents, membrane, hrp substrate necessary for a perfect blot. however, the kit components are also sold separately.

key features easy to perform:this kit has fewer and simpler steps than other western kits. low background:the kit contains westcleartmnitrocellulose membrane and lumisensortmseries of chemiluminescent hrp substrate kits optimized for low background. reproducible results:the kit produces highly reproducible results. no additional secondary antibodyis needed. examples:

comparison of one-hour westerntmblot detection with classical western blot detection.
one-hour westerntmblot detection was compared with classical western blot detection of α-tubulin protein from hela cell lysate. serially diluted hela cell lysate samples were western blotted to westcleartmnitrocellulose membrane (genscript, l00224) after sds-page. the membrane was then cut into two halves and processed with different procedures using α-tubulin monoclonal antibody (abcam, ab7291): one-hour westerntmblot detection (1 hr, right panel of figure 2) and classical western-blot detection (4.5 hours, left panel of figure 2).

figure 2. western blots for the detection of α-tubulin protein from hela cell lysate by both classical western and one-hour westerntmbasic kit. 10 μg, 2.5 μg, 0.63 μg, and 0.16 μg of hela cell lysate (bd biosciences, 611449) were loaded in different lanes as shown in the figure. the classical western blot was developed with ecl system (ge healthcare, rpn2109). the one-hour western blot was developed with lumisensortmchemiluminescent hrp substrate kit (genscript, l00221v60).

technical manual: download pdf filetm0352 components componentslumisensortmchemiluminescent hrp substratepretreat solution apretreat solution buser manualwb-1 solutionwb-2 solutionwestcleartmnitrocellulose membrane
(0.2 μm, 7.5 x 8 cm)5x wash solutionchromosensortmone solution tmb substrate

l00204	l00205	l00399
x	x	x
50 ml	50 ml	50 ml
50 ml	50 ml	50 ml
1	1	1
0.5 ml	0.5 ml	0.5 ml
50 ml	50 ml	50 ml
x	x	x
125 ml	125 ml	125 ml
x	x	x

document msds:20060818160706(pdf) related products name selected

l00204t	one-hour westerntmstandard kit using tmb (rabbit)	1kit (5 assays)	$75.00
l00205t	one-hour westerntmstandard kit using tmb (mouse)	1kit (5 assays)	$75.00
l00224a60	westcleartmnitrocellulose membrane	1pk (5 sheets of 7.5 x 8 cm)	$20.00
l00221v60	lumisensortmchemiluminescent hrp substrate kit	1kit (2 x 30ml)	$55.00
l00222v60	chromosensortmone-solution tmb substrate	1pk (60ml)	$28.00
l00228	one-hour westerntmstandard kit (goat)	1kit (5 assays)	$75.00
l00205	one-hour westerntmbasic kit (mouse)	1kit (5 assays)	$60.00
l00204c	one-hour westerntmstandard kit (rabbit)	1kit	$75.00
l00205c	one-hour westerntmstandard kit (mouse)	1kit	$75.00
l00399	one-hour westerntmbasic kit (goat)	1kit (5 assays)	$60.00