Enzymatics-- 末端修复混合液(低浓度)--Y9140-LC-L
末端修复混合液(低浓度)
摘要:针对低浓度DNA,如普通克隆的优化
产品描述:
The End-Repair mix converts DNA containing damaged or incompatible 5′- and/or 3′-protruding ends to 5′-phosphorylated, blunt-ended DNA. This high-concentration formulation of the End-Repair Mix is compatible with applications requiring >1 microgram of DNA to be prepared for blunt-end ligation. The conversion to blunt-ended DNA is accomplished by exploiting the 5′→3′ polymerase and 3′→5′ exonuclease activities of T4 DNA Polymerase (P7080). T4 Polynucleotide Kinase (Y9040) ensures that the ends of the blunt-ended DNA fragments are 5′-phosphorylated for subsequent ligation by T4 DNA ligase(L6030-HC)z
产品组分
Supplied in: 10mM Tris-HCl, 100 mM KCl, 1mM DTT, 0.1mM EDTA, 0.1% Triton X-100, 50% glycerol pH 7.4 @ 25⁰C. Supplied with:
10X End-Repair Buffer (B9140): 1M Tris-HCl, 500mM NaCl, 100mM MgCl2, 50mM DTT, 0.25%
产品信息:
Part Number Y9140-LC-L
Concentration 1 Rxn/μL
Unit Size 200 Reactions
产品特征:
Storage Temperature -25⁰C to -15⁰C
纯度:>99%
E.coli DNA Contamination:<10 copies