末端修复混合液(低浓度)
更新时间:2015-08-12 17:38:28 信息编号:3834692 发布者IP:113.109.36.101 浏览:164次- 供应商
- 广州胜创生物科技有限公司 商铺
- 认证
- 资质核验:已通过营业执照认证入驻顺企:第9年主体名称:广州胜创生物科技有限公司组织机构代码:440106000367748
- 报价
- 请来电询价
- 品牌
- Enzymatics
- 型号
- Y9140-LC-L
- 关键词
- Enzymatics
- 所在地
- 广州市天河区长兴路291号901房(该场所仅限办公用途)
- 联系电话
- 4006-400-850
- 手机号
- 13570444143
- 办公室文员
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产品详细介绍
Enzymatics-- 末端修复混合液(低浓度)--Y9140-LC-L
末端修复混合液(低浓度)
摘要:针对低浓度DNA,如普通克隆的优化
产品描述:
The End-Repair mix converts DNA containing damaged or incompatible 5′- and/or 3′-protruding ends to 5′-phosphorylated, blunt-ended DNA. This high-concentration formulation of the End-Repair Mix is compatible with applications re >1 microgram of DNA to be prepared for blunt-end ligation. The conversion to blunt-ended DNA is accomplished by exploiting the 5′→3′ polymerase and 3′→5′ exonuclease activities of T4 DNA Polymerase (P7080). T4 Polynucleotide Kinase (Y9040) ensures that the ends of the blunt-ended DNA fragments are 5′-phosphorylated for subsequent ligation by T4 DNA ligase(L6030-HC)z
产品组分
Supplied in: 10mM Tris-HCl, 100 mM KCl, 1mM DTT, 0.1mM EDTA, 0.1% Triton X-100, 50% glycerol pH 7.4 @ 25⁰C. Supplied with:
10X End-Repair Buffer (B9140): 1M Tris-HCl, 500mM NaCl, 100mM MgCl2, 50mM DTT, 0.25%
产品信息:
Part Number Y9140-LC-L
Concentration 1 Rxn/μL
Unit Size 200 Reactions
产品特征:
Storage Temperature -25⁰C to -15⁰C
纯度:>99%
E.coli DNA Contamination:<10 copies
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