TwistAmp Basic RT试剂盒
| 产品名称 |
TwistAmp Basic RT试剂盒 |
| 公司名称 |
畅宇云商（北京）科技有限公司 |
| 价格 |
面议 |
| 规格参数 |
品牌:TwistDX
型号:Basic RT
货号:TABSRT01KIT |
| 公司地址 |
北京市门头沟区石龙经济开发区永安路20号3号楼A-7427室 |
| 联系电话 |
13810792952 13810792952 |
产品详情
|
TABSRT01KIT
|
TwistAmp™ Basic RT
|
用于逆转录RPA反应
|
产品描述：
TwistAmp® 核酸扩增产品，能够在15分钟内进行常温下的单分子核酸检测该技术对硬件设备的要求很低，特别适合用于体外诊断、兽医、食品安全、生物安全、农业等领域。TwistAmp Basic用于逆转录反应，CDNA快速扩增. 除基本组分外, 还加有通用的逆转录酶，但不含Rnase抑制剂。
Basic Information
RPA
1) Primers must be 30-35 bases
2) Works best at constant temperature (40-42°C)
3) Amplicons of 80-400bp are
Set-up (single-plex)1
1) Prepare reaction mix in 1.5ml tube:
Primer A (10μM) 2.4 μl
Primer B (10μM) 2.4 μl
Rehydration Buffer 29.5 μl
Template,
RNase Inhibitor and dH2O 13.2 μl
(Total Volume 47.5 μl)
Vortex and spin briefly
2) Add reaction mix to freeze-dried reaction. Pipette to mix.
3) Add 2.5 μl of 280mM MgAc (supplied) and mix well to start reaction.
4) Incubate at 40-42°C for 20-40 minutes.
4b) For low template copy number,
remove strip after 5-7 minutes, invert
vigorously 8-10 times to mix & spin
briefly, replace in heating device.
5) After 20-40 minutes, clean amplicons before running on agarose gels.
WARNING: RPA REACTIONS START AT ROOM TEMPERATURE AS SOON AS
MAGNESIUM IS ADDED.