| 产品名称 | X-Gal/IPTG Premix |
|---|---|
| 公司名称 | 北京华夏远洋科技有限公司 |
| 价格 | .00/个 |
| 规格参数 | 主要组成成分:X-Gal/IPTGPremix 性状:试剂 适应症:科研试验 |
| 公司地址 | 北京市丰台区造甲街110号2号平房(新村企业集中办公区) |
| 联系电话 | 010-62898668 15201195988 |
| 主要组成成分 | X-Gal/IPTG Premix | 性状 | 试剂 |
| 适应症 | 科研试验 | 规格 | 5ml |
| 有效期 | 36（月） | 生产企业 | genscript |
| 药（械）准字 | genscript M00092 |
l00204 | one-hour westerntmbasic kit (rabbit) | 1kit (5 assays) | $60.00 | |
genscript"s one-hour westerntmkit yields journal-quality western or dot blot results in about one hour. using genscript"s breakthrough immunodetection technology (patent pending), the kit replaces the classical three-step western process, which take nearly five hours. transfer the proteins from gel to membrane and incubate it in the pretreat solution for five minutes. then incubate in wb-2 solution with the mixture of primary antibody and wb-1 solution for 40 minutes. then wash three times for five or ten minutes each. the membrane can then be developed with the hrp substrate. the one-hour westerntmprocedure is contrasted with a classical three-step western at right.
the one-hour westerntmkit is designed to produce high signal with low background for quick and clear western analysis of proteins.
except for basic kits, all other kits contain all the necessary reagents, buffers and materials such as membrane for performing a western blot, and no additional secondary antibody is needed.
the one-hour westerntmkit saves precious research time. for added cost-efficiency, we recommend our complete kits, which contain all the reagents, membrane, hrp substrate necessary for a perfect blot. however, the kit components are also sold separately.
comparison of one-hour westerntmblot detection with classical western blot detection.
one-hour westerntmblot detection was compared with classical western blot detection of α-tubulin protein from hela cell lysate. serially diluted hela cell lysate samples were western blotted to westcleartmnitrocellulose membrane (genscript, l00224) after sds-page. the membrane was then cut into two halves and processed with different procedures using α-tubulin monoclonal antibody (abcam, ab7291): one-hour westerntmblot detection (1 hr, right panel of figure 2) and classical western-blot detection (4.5 hours, left panel of figure 2).
figure 2. western blots for the detection of α-tubulin protein from hela cell lysate by both classical western and one-hour westerntmbasic kit. 10 μg, 2.5 μg, 0.63 μg, and 0.16 μg of hela cell lysate (bd biosciences, 611449) were loaded in different lanes as shown in the figure. the classical western blot was developed with ecl system (ge healthcare, rpn2109). the one-hour western blot was developed with lumisensortmchemiluminescent hrp substrate kit (genscript, l00221v60).
| l00204 | l00205 | l00399 |
| x | x | x |
| 50 ml | 50 ml | 50 ml |
| 50 ml | 50 ml | 50 ml |
| 1 | 1 | 1 |
| 0.5 ml | 0.5 ml | 0.5 ml |
| 50 ml | 50 ml | 50 ml |
| x | x | x |
| 125 ml | 125 ml | 125 ml |
| x | x | x |
l00204t | one-hour westerntmstandard kit using tmb (rabbit) | 1kit (5 assays) | $75.00 | |
l00205t | one-hour westerntmstandard kit using tmb (mouse) | 1kit (5 assays) | $75.00 | |
l00224a60 | westcleartmnitrocellulose membrane | 1pk (5 sheets of 7.5 x 8 cm) | $20.00 | |
l00221v60 | lumisensortmchemiluminescent hrp substrate kit | 1kit (2 x 30ml) | $55.00 | |
l00222v60 | chromosensortmone-solution tmb substrate | 1pk (60ml) | $28.00 | |
l00228 | one-hour westerntmstandard kit (goat) | 1kit (5 assays) | $75.00 | |
l00205 | one-hour westerntmbasic kit (mouse) | 1kit (5 assays) | $60.00 | |
l00204c | one-hour westerntmstandard kit (rabbit) | 1kit | $75.00 | |
l00205c | one-hour westerntmstandard kit (mouse) | 1kit | $75.00 | |
l00399 | one-hour westerntmbasic kit (goat) | 1kit (5 assays) | $60.00 | |