| 产品名称 | Gα13-GTP 小鼠单抗丨Gα13抗体丨免疫荧光 |
|---|---|
| 公司名称 | 武汉费斯德生物科技有限公司 |
| 价格 | 4800.00/个 |
| 规格参数 | 品牌:NewEast Bio 货号:26902 规格:30 微升 |
| 公司地址 | 武汉市江汉区前进五路97号5栋1层8号 |
| 联系电话 | 027-63497286 18162422647 |
货号: 26902 规格: 30 L 基因符号: Gna13 描述: Anti-Gα13-GTP Mouse Monoclonal Antibody 背景介绍: Heterotrimeric G proteins are essential cellular signal transducers. Gα13 is one of the G proteins that could mediate cell migration and angiogenesis. Other biochemical and physiological functions of Gα13 are being explored. 免疫原: Recombinant full length active Gα13 protein 经过测试的应用: IP, IHC, IF 推荐稀释比:
IP: 1 g for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250浓度: 1 mg/ml 宿主: Mouse 形式: Liquid 克隆性: Monoclonal 亚型: IgG2b 纯度: Purified from ascites 防腐剂: No 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol 种属反应性: Anti-active Gα13 monoclonal antibody recognizes active Gα13 of vertebrates. 储存条件: Store at -20°C. Avoid repeated freezing and thawing 免疫沉淀/蛋白质印迹:
IP/WB analysis of active Gα13 proteins. Purified Gα13 proteins were treated with GDP (lane 1) or GTPGTPγS (lane 2). After immunoprecipitation with anti-active Gα13-GTP mouse monoclonal antibody (Cat. # 26902), these proteins were separated by SDS/PAGE and western blotted with anti- Gα13 mouse monoclonal antibody (Cat. # 26004).Immunohistochemistry:
Staining of MEF cells (treated with PDGF) with anti- Gα13-GTP monoclonal antibody showing the localization of Gα13-GTP in the leading edge of migrating cells.