TwistAmp exo试剂盒

TAEXO02KIT

TwistAmp™ exo

用于real-time探针检测

产品描述：

TwistAmp® 核酸扩增产品，能够在15分钟内进行常温下的单分子核酸检测该技术对硬件设备的要求很低，特别适合用于体外诊断、兽医、食品安全、生物安全、农业等领域。TwistAmp exo 用于real-time探针检测. 除基本组分外, 还含有内切酶3，减少扩增产物的末端拷贝量，所以不能用于电泳分析，但在RPA系统中产生更强的动态荧光信号，便于实时定量。 Basic Information RPA 1) Primers must be 30-35 bases 2) Works best at constant temperature (37-39°C) 3) Amplicons of 80-400bp are preferred 4) TwistAmp® exo Probe required - see overleaf Set-up (single-plex)1 1) Prepare reaction mix in 1.5ml tube: Primer A (10μM)    2.1 μl Primer B (10μM)    2.1 μl TwistAmp® exo Probe (10μM)    0.6 μl Rehydration Buffer   29.5 μl Template and dH2O   13.2 μl (Total Volume   47.5 μl) Vortex and spin briefly 2) Add reaction mix to freeze-dried reaction. Pipette to mix. 3) Add 2.5 μl of 280mM MgAc (supplied) and mix well to start reaction. 4) Place strip in Twista® and start run: 37-39°C, 20 minutes. 4b) For low template copy number, remove strip after 4 minutes, vortex & spin briefly, replace in Twista®. WARNING: RPA REACTIONS START AT ROOM TEMPERATURE AS SOON AS