Anti-Digoxigenin-POD
更新时间:2016-12-01 10:48:54 信息编号:4442952 发布者IP:103.44.204.202 浏览:87次![](http://img4.11467.com/u/23565750/194528941.jpg)
- 供应商
- 北京冬璞泰和科技有限责任公司 商铺
- 认证
- 资质核验:已通过营业执照认证入驻顺企:第9年主体名称:北京冬璞泰和科技有限责任公司组织机构代码:911101080649175474
- 报价
- 请来电询价
- 品牌
- 罗氏
- 型号
- 11207733910
- 关键词
- 11207733910 罗氏
- 所在地
- 北京市海淀区瀚河园131号
- 联系电话
- 010-57181378
- 手机号
- 13716040241
- 联系人
- 马浩然 请说明来自顺企网,优惠更多
产品详细介绍
Use Anti-Digoxigenin-POD, Fab fragments for the detection of
digoxigenin-labeled compounds using: • Dot blot
• ELISA
• Immunohistocytochemistry
• In situ hybridization
• Western blot
Fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to horseradish peroxidase.
Other Notes仅用于生命科学研究。不可用于诊断。
Preparation NoteAfter immunization with digoxigenin, sheep IgG was purified by
ion-exchange chromatography, and the specific IgG was isolated by
immunosorption. The Fab fragments obtained by papain digestion were
purified by gel filtration, conjugated to the specific label, and
stabilized in buffer.
Working concentration: Working concentration of conjugate
will depend on the application and substrate. The following
concentrations should be taken as a guideline: Dot blot: 150
mU/ml
• ELISA: 50 to 150 mU/ml
• Immunohistocytochemistry: 250 to 500 mU/ml
• In situ hybridization: 1.5 to 7.5 U/ml
• Southern blot: 150 mU/ml
• Western blot: 250 to 500 mU/ml
Working solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf
serum (v/v) or sheep normal serum can be used for reduction of
unspecific binding.
Add 1 ml double-distilled water to a final concentration of 150 U/ml.
SpecificityThe polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.
biological source | from sheep |
clone | polyclonal |
form | lyophilized (clear, brown solution after reconstitution) |
mfr. no. | Roche |
packaging | pkg of 150 U |
conjugate | peroxidase conjugate |
shipped in | wet ice |
storage temp. | 2-8°C |
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