Anti-Digoxigenin-POD

Use Anti-Digoxigenin-POD, Fab fragments for the detection of digoxigenin-labeled compounds using: • Dot blot
• ELISA
• Immunohistocytochemistry
• In situ hybridization
• Western blot
 

Fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to horseradish peroxidase.

仅用于生命科学研究。不可用于诊断。

After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.

Working concentration: Working concentration of conjugate will depend on the application and substrate. The following concentrations should be taken as a guideline: Dot blot: 150 mU/ml
• ELISA: 50 to 150 mU/ml
• Immunohistocytochemistry: 250 to 500 mU/ml
• In situ hybridization: 1.5 to 7.5 U/ml
• Southern blot: 150 mU/ml
• Western blot: 250 to 500 mU/ml

Working solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.

Add 1 ml double-distilled water to a final concentration of 150 U/ml.

The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.