millipore amicon ultra-2 ml centrifugal filters for dna and protein purification and concentration
密理博amicon ultra-2ml超滤离心管
订货信息
货号和规格 |
|||
货号 |
品名 |
nmwl (kda) |
包装 (支/包) |
ufc200324 |
amicon ultra-2 centrifugal filter unit with ultracel-3 membrane |
3 |
24 |
ufc201024 |
amicon ultra-2 centrifugal filter unit with ultracel-10 membrane |
10 |
24 |
ufc203024 |
amicon ultra-2 centrifugal filter unit with ultracel-30 membrane |
30 |
24 |
ufc205024 |
amicon ultra-2 centrifugal filter unit with ultracel-50 membrane |
50 |
24 |
ufc210024 |
amicon ultra-2 centrifugal filter unit with ultracel-100 membrane |
100 |
24 |
概述
concentrates 2 ml down to 15-70 µl 98% sample recovery fast processing time - 10 to 30 minute spin times engineered dead stop reverse spin capability provides consistent recoveries
applications
concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms purification of macromolecular components found in tissue culture extracts or cell lysates primer removal, pcr cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to hplc desalting, buffer exchange and protein dialysis size exclusion and protein fractionationmaterial supplied
the amicon ultra-2 device is supplied with two microcentrifuge tubes. during operation, one tube is used to collect filtrate; the other tube is to recover the concentrated sample.
规格
volumes |
|
maximum initial sample volume, µl |
2.0 |
typical final concentration volume, µl |
15 – 70 |
optimal and maximum relative centrifugal force – swinging bucket rotor |
4,000 x g for concentration spin |
optimal and maximum relative centrifugal force – fixed angle rotor |
7,500 x g for concentration spin |
active membrane area, cm2 |
1 |
hold up volume, µl |
< 5 |
materials |
|
filter device |
copolymer styrene/butadiene |
membrane |
millipore ultracell low-binding regenerated cellulose |
collection tube |
polypropylene |
the ultrafiltration membranes in amicon ultra-2 ml devices contain trace amounts of the following: |
amicon ultra 3k, 10k, 30k, 50k, 100k devices: glycerine |
if these materials interfere with analysis, pre-rinse the device with buffer or milli-q water. if interference continues, rinse with 0.1 n naoh followed by a second spin of buffer or milli-q water. |
|
dimensions |
|
filter device, mm (in.) |
diameter: 15.9 (0.63) |
length: 70.7 (2.78) |
|
tube, mm (in.) |
diameter: 13.8 (0.54) |
length: 52.9 (2.08) |
|
filter device and tube, mm (in.) |
length (concentration mode; device in tube): 13.7 (0.54) |
length (reverse spin; device upside down in tube): 34.5 (1.36) |