lps (lipopolysaccharide) triggers a cascade of immunostimulatory
and toxic
pathophysiological events, including peripheral vascular collapse
by releasing endotoxin initiate septic shock.
generally, the hot phenol-water extraction method is usually used
for extracting lps from gram-negative bacteria. however, it is
complicated and takes long time to extract.
the intron lps extraction kit is the first commercially product for
extracting lps from bacteria. lps extraction kit is designed for
rapid and convenient extraction of lps from bacterial
cells.
lps extraction from bacterial cells
distribution of bacteria by patterns
of the carbohydrate chain length
immunostimulatory effect of the
extracted lps from bacteria
very simple and convenient
steps
rapid reaction time (within 60
minutes)
format
: soution type (lysis buffer and
purification buffer)
sample
source: bacterial cells
sample
size: the yield of lps extraction is proportional to
volume of culture. the yield of lps is at its maximum when 5ml of
culture is used. the optimal culture volume is 5ml at od600 of
0.8-1.2.
prep.
time: <60 minutes including a
lysis step
rehydration
volume: 50-200ml
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