ONE-HOUR WesternTM Standard Kit (Mouse)

主要组成成分	ONE-HOUR WesternTM Standard Kit (Mouse)	性状	试剂盒
适应症	科研试验	规格	1 Kit(5 Assays)
生产企业	GenScript	药（械）准字	GenScript L00205C

name selected

l00205c	one-hour westerntmstandard kit (mouse)	1kit	680.00

one-hour westerntmstandard kit (mouse) kit description

genscript"s one-hour westerntmkit yields journal-quality western or dot blot results in about one hour. using genscript"s breakthrough immunodetection technology (patent pending), the kit replaces the classical three-step western process, which can take nearly five hours. transfer the proteins from gel to membrane and incubate it in the pretreat solution for five minutes. then incubate in wb-2 solution with the mixture of primary antibody and wb-1 solution for 40 minutes. then wash three times for five or ten minutes each. the membrane can then be developed with the hrp substrate. the one-hour westerntmprocedure is contrasted with a classical three-step western at right.

the one-hour westerntmkit is designed to produce high signal with low background for quick and clear western analysis of proteins.

except for basic kits, all other kits contain all the necessary reagents, buffers and materials such as membrane for performing a western blot, and no additional secondary antibody is needed.

the one-hour westerntmkit saves precious research time. for added cost-efficiency, we recommend our standard kits, which contain all the reagents, membrane, hrp substrate necessary for a perfect blot. however, the kit components are also sold separately.

key features easy to perform:this kit has fewer and simpler steps than other western kits. low background:the kit contains westcleartmnitrocellulose membrane and lumisensortmseries of chemiluminescent hrp substrate kits optimized for low background. reproducible results:the kit produces highly reproducible results. no additional secondary antibodyis needed. examples:

comparison of the two one-hour westerntmblot kits of different sensitivities using monoclonal antibodies:
two similar blots were processed with the same procedures using different one-hour westerntmkits: standard (l00205c) and advanced (l00242). 10 µg and 2.5 µg of thetmanti-gst monoclonal antibody (mouse) (genscript, a00865), respectively, were used with these two kits to detect gst protein.
the results are shown in figure 1.

figure 1. western blots for the detection of gst protein by one-hour westerntmusing different one-hour westerntmkits: standard (l00205c) and advanced (l00242). 33.0, 11.0, 3.60, 1.20, 0.41, 0.13 and 0.045 ng of gst protein were loaded into lanes 1, 2, 3, 4, 5, 6, and 7 respectively.

comparison of the two one-hour westerntmblot kits of different sensitivities using polyclonal antibodies:
two similar blots were processed with the same procedures using different one-hour westerntmkits: standard (l00204c) and advanced (l00241). 10 µg and 2.5 µg of rabbit anti-gst-tag polyclonal antibody (genscript, a00097), respectively, were used with the two kits to detect gst protein.
the results are shown in figure 2.

figure 2. western blots for the detection of gst protein by one-hour westerntmusing different one-hour westerntmkits: standard (l00204c) and advanced (l00241). 25.0, 12.5, 6.25, 3.10, 1.50, and 0.78 ng of gst protein were loaded into lanes 1, 2, 3, 4, 5, and 6 respectively.

technical manual: download pdf filetm0352 components componentslumisensortmchemiluminescent hrp substratepretreat solution apretreat solution buser manualwb-1 solutionwb-2 solutionwestcleartmnitrocellulose membrane
(0.2 μm, 7.5 x 8 cm)5x wash solutionchromosensortmone solution tmb substrate

l00204t	l00204c	l00228t	l00205t	l00205c	l00228
x	2 x 7.5 ml	x	x	2 x 7.5 ml	2 x 7.5 ml
50 ml	50 ml	50 ml	50 ml	50 ml	50 ml
50 ml	50 ml	50 ml	50 ml	50 ml	50 ml
1	1	1	1	1	1
0.5 ml	0.5 ml	0.5 ml	0.5 ml	0.5 ml	0.5 ml
50 ml	50 ml	50 ml	50 ml	50 ml	50 ml
5 sheets	5 sheets	5 sheets	5 sheets	5 sheets	5 sheets
125 ml	125 ml	125 ml	125 ml	125 ml	125 ml
15 ml	x	15 ml	15 ml	x	x

document msds:20060818160706(pdf) references zhang w, et al. transcriptional down-regulation of thromboxane a(2) receptor expression via activation of mapk erk1/2, p38/nf-kappab pathways. j. vasc. res. 2009;46(2):162-74. related products name selected

l00204c	one-hour westerntmstandard kit (rabbit)	1kit	$75.00
l00204	one-hour westerntmbasic kit (rabbit)	1kit (5 assays)	$60.00
l00205	one-hour westerntmbasic kit (mouse)	1kit (5 assays)	$60.00
l00228	one-hour westerntmstandard kit (goat)	1kit (5 assays)	$75.00